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Normal phase liquid chromatography-electrospray ionization tandem mass spectrometry analysis of phospholipid molecular species in blood mononuclear cells: application to cystic fibrosis.

Malavolta M, Bocci F, Boselli E, Frega NG

Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, via Brecce Bianche, 60131 Ancona, Italy.

The use of HPLC coupled on-line with a mass spectrometer is a very powerful tool in order to analyze intact PLs molecular species (PMS) without the need of derivatization, thus decreasing the risk of artifacts formation. A normal-phase HPLC-ESI-MS-MS method has been developed in order to study the human blood mononuclear cell PMS composition. This method was applied to characterize PMS from seven CF subjects and from seven age-matched healthy subjects. More than 140 phospholipid molecular species from phosphatidylethanolamine (PE), plasmalogen phosphatidylethanolamine (pPE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC) and sphingomyelin (Sph) were identified and compared. Differences between the two groups were found in pPE (p16:0/22:6), pPE (p18:0/22:6), PE (16:0/20:4) and PC (16:0/18:2) which were significantly lower in CF subjects and in PC (16:0/16:1) which was significantly higher in CF subjects.

Published 21 September 2004 in J Chromatogr B Analyt Technol Biomed Life Sci, 810(2): 173-86.
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